Tolerance of <i>Chlorella ellipsoidea</i> and Its Removal of Heavy Meta

Abstract

分离筛选获得了一株高重金属抗性的椭圆小球藻(Chlorella ellipsoidea),并研究了不同浓度的重金属铜、锌、镍、镉对该藻生长和叶绿素a含量的影响及其对重金属离子的吸收富集作用.结果显示,该藻对Zn²⁺和Cd²⁺具有很高的耐受性.对四种重金属的耐受能力依次为锌 > 镉 > 镍 > 铜.其叶绿素a含量与重金属离子浓度呈明显负相关.该藻对重金属具有很好的去除效果,经15μmol/L Cu²⁺、300μmol/L Zn²⁺、100μmol/L Ni²⁺、30μmol/L Cd²⁺浓度72h处理,去除率分别达到40.93%、98.33%、97.62%、86.88%,可应用于含重金属废水的处理;A st rain of Chlorella ellipsoidea with high tolerance to heavy metals was isolated. Grow th and heavy metals removal were studied on this alg a exposed to different heavy metal concent rations.The ef fect of heavy metal on grow th indicated that cells can live in higher Zn²⁺ and Cd²⁺ concent rations.The tolerant sequence to 4 kinds of metals for C.ell ipsoidea was Zn²⁺ > Cd²⁺ > Ni²⁺ > Cu²⁺.The content of chlo rophyll a decreased with the increase of heavy metal concent rations. In this ex periment, C.ellipsoidea also show ed its rather st rong ability to remove heavy metals from the medium.After 72-hour treatment, the removal rates of metals were 40.93%, 98.33%, 97.62%and 86.88% respectively, for 15 μmol/L Cu²⁺, 300μmol/L Zn²⁺, 100μmol/L Ni²⁺ and 30μmol/L Cd²⁺.These results suggested that the C.ellipsoidea strain could be applied to the treatment of heavy metal wastewater.