Tolerance of crambe (Crambe abyssinica Hochst) to salinity and water stress during seed germination and initial seedling growth

Luiz Antônio dos Santos Dias,Laércio Junio da Silva,Hugo Tiago Ribeiro Amaro,Eduardo Fontes Araújo,Martha Freire da Silva,Denise Cunha Fernandes dos Santos Dias

doi:10.1590/1413-7054201943025418

Luiz Antônio dos Santos Dias, Laércio Junio da Silva + Show 4 more

Open Access

https://doi.org/10.1590/1413-7054201943025418

Copy DOI

Abstract

ABSTRACT Crambe is an oilseed plant whose oil is suitable for various industrial purposes, such as production of biodiesel, plastics, rubbers, and cosmetics. There are reports of the rusticity of this species and its potential for growth in arid and semi-arid environments, where water scarcity and soil salinity are common. However, studies regarding the tolerance of its seeds to these stresses and the physiological responses triggered during germination and seedling establishment under these conditions are incipient. The objective of this study was to evaluate the tolerance of crambe seeds to water deficit and salt stress during germination and early seedling development, as well as to investigate the role of antioxidant enzymes on tolerance to these conditions. The seeds were exposed to water and salt stresses and their performance was analyzed. Seed water content, imbibition rate, percentage and speed of germination, and the base water potential for seed germination were evaluated. In the seedlings, the following measurements were made: root and shoot length, fresh and dry matter of roots and shoots, moisture content, and activity of the enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX). Water deficit and salt stress reduce the water uptake rate of the seeds, the germination and growth of seedlings. The crambe seeds had higher tolerance to salt stress than to water deficit. Absorption of salt and the action of the SOD and CAT antioxidant enzymes are part of the tolerance mechanisms of crambe seeds to salt stress.

Highlights

Crambe (Crambe abyssinica Hochst) is an annual, short-cycle oilseed, belonging to the Brassicaceae family, which stands out for good yield and high oil content in its seeds
It is a very promising crop for industrial uses; the oil from its seeds can be used in production of biofuels, biodegradable plastics, nylon, adhesives, electrical isolation, lubricants, anticorrosive agents, rubbers, and cosmetics (Falasca et al, 2010; Tavares et al, 2017)
The bran produced in the oil extraction process can be used for animal feed (Falasca et al, 2010; Goes et al, 2018), and the leaves used in the preparation of nematicides (Coltro-Roncato et al, 2016)

Summary

Introduction

Crambe (Crambe abyssinica Hochst) is an annual, short-cycle oilseed, belonging to the Brassicaceae family, which stands out for good yield and high oil content in its seeds. Seed oil content is about 36%-38% (Pitol; Broch; Roscoe, 2010). It is a very promising crop for industrial uses; the oil from its seeds can be used in production of biofuels, biodegradable plastics, nylon, adhesives, electrical isolation, lubricants, anticorrosive agents, rubbers, and cosmetics (Falasca et al, 2010; Tavares et al, 2017). There are reports of the rusticity of this species and its potential for growing in arid and semi-arid environments, where water scarcity and soil salinity are common (Ionov et al, 2013; Pivetta et al, 2016), which could make it an excellent crop alternative under these conditions. Water and salt stresses are among the abiotic factors that most affect seed germination and plant development. Understanding seed responses to these stresses is important in the face of global climate change and increasing salinization of soils

Objectives

Methods

Results