Tolerance of chufa (Cyperus esculentus L.) plants, representing the higher plant compartment in bioregenerative life support systems, to super-optimal air temperatures

Abstract

Plants intended to be included in the photosynthesizing compartment of the bioregenerative life support system (BLSS) need to be studied in terms of both their production parameters under optimal conditions and their tolerance to stress factors that might be caused by emergency situations. The purpose of this study was to investigate tolerance of chufa (Cyperus esculentus L.) plants to the super-optimal air temperature of 45±1°C as dependent upon PAR (photosynthetically active radiation) intensity and the duration of the exposure to the stress factor. Chufa plants were grown hydroponically, on expanded clay, under artificial light. The nutrient solution was Knop’s mineral medium. Until the plants were 30days old, they had been grown at 690μmolm−2s−1 PAR and air temperature 25°C. Thirty-day-old plants were exposed to the temperature 45°C for 6h, 20h, and 44h at PAR intensities 690μmolm−2s−1 and 1150μmolm−2s−1. The exposure to the damaging air temperature for 44h at 690μmolm−2s−1 PAR caused irreversible damage to PSA, resulting in leaf mortality. In chufa plants exposed to heat shock treatment at 690μmolm−2s−1 PAR for 6h and 20h, respiration exceeded photosynthesis, and CO2 release in the light was recorded. Functional activity of photosynthetic apparatus, estimated from parameters of pulse-modulated chlorophyll fluorescence in Photosystem 2 (PS 2), decreased 40% to 50%. After the exposure to the stress factor was finished, functional activity of PSA recovered its initial values, and apparent photosynthesis (Papparent) rate after a 20-h exposure to the stress factor was 2.6 times lower than before the elevation of the temperature. During the first hours of plant exposure to the temperature 45°C at 1150μmolm−2s−1 PAR, respiration rate was higher than photosynthesis rate, but after 3–4h of the exposure, photosynthetic processes exceeded oxidative ones and CO2 absorption in the light was recorded. At the end of the 6-h exposure, Papparent rate was close to that recorded prior to the exposure, and no significant changes were observed in the functional activity of PSA. At the end of the 20-h exposure, Papparent rate was close to its initial value, but certain parameters of the functional activity of PSA decreased 25% vs. their initial values. During the repair period, the parameters of external gas exchange recovered their initial values, and parameters of pulse-modulated chlorophyll fluorescence were 20–30% higher than their initial values. Thus, exposure of chufa plants to the damaging temperature of the air for 20h did not cause any irreversible damage to the photosynthetic apparatus of plants at either 690μmolm−2s−1 or 1150μmolm−2s−1 PAR, and higher PAR intensity during the heat shock treatment enhanced heat tolerance of the plants.