ToF‐SIMS investigations of adsorbed proteins on dental titanium

Abstract

AbstractTwo model proteins, bovine serum albumin (BSA) and lysozyme (LSZ), were used to study adsorption behavior on dental titanium by time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS). Protein films were prepared as single component layers and as mixtures of the two proteins. Additionally, the behavior of consecutive adsorption of both proteins was investigated. The obtained data were analyzed by means of principal component analysis (PCA). Dental titanium samples with a layer consisting of simply one protein could be separated from each other by selecting amino acid related peaks and using PCA or samples coated with a mixture of both proteins, the differentiation was also possible. The partial displacement of LSZ molecules by BSA was shown in the case of simultaneous adsorption. The consecutive adsorption of BSA and subsequently LSZ resulted in layers with a bigger amount of LSZ than of BSA compared to the layers obtained by simultaneous adsorption.