Tocotrienol supplementations ameliorate oxidative stress in thalamus of rats exposed to low-to-moderate doses of ethanol

Abstract

Background: Functional deterioration of thalamic structures has been reported in many occasions of ethanol-induced neurodegeneration. Neuroprotective role of Tocotrienol (T3) is well established along with its antioxidant property. Interaction of oral T3 supplementation modalities with the thalamic oxidative stress parameters were studied in low-to-moderate doses of ethanol (Et) exposures. Materials and Methods: Four phase of experiments were carried out with nil (Et-0) and three doses of Et exposures (Et-I, Et-II and Et-III) for 4 weeks. In each phase, 4 groups of Wistar rats were maintained with sham supplementation (NT3), prior supplementation (PT3), simultaneous supplementation (ST3) and total supplementation (TT3) with T3 for 6 weeks. Thalamic levels of reduced glutathione (GSH) and lipid peroxidation (LPO) were estimated. Results: Two-way ANOVA demonstrated that all the thalamic GSH and LPO were significantly influenced by the modalities of T3 supplementation. However, low-to-moderate doses of Et exposures contributed significantly in alterations of only LPO level of thalamus. Conclusion: Out of the tested modalities, best protection in terms of oxidative stress was observed when prior and simultaneous supplementation modalities were combined (TT3).