Tobravirus isolation and RNA extraction.

Abstract

AbstractThe tobraviruses have a genome consisting of two positive-sense, single-stranded RNA molecules. The two genomic RNAs, RNA-1 and RNA-2, are encapsidated separately in rod-shaped particles with lengths of 180–215 nm (L particles) and 46–115 nm (S particles), respectively (Fig. 1) (1,2). Both the RNAs are encapsidated by a single species of coat protein (CP) with a mol wt of approx 23 kDa. Tobraviruses can be divided into three serologically distinct subgroups: tobacco rattle virus (TRV), which is the type member, pea early browning virus (PEBV), and pepper ringspot virus (PRV). In the field, these viruses are transmitted by soil-inhabiting nematodes of the family Trichodoridae, and certain isolates are also transmitted through the seed. Tobraviruses can infect a wide range of plant species including economically important crops such as potatoes and ornamental bulbs. Tobravirus structure and genome organization. (ai) Genome organization of RNA-1 of TRV isolate SYM. (aii) Genome organization of RNA-2 of various TRV isolates. (bi and bii) Genome organization of RNA-1 and RNA-2 of PEBV isolate SP5. RNA is shown as a horizontal line and the positions of the open reading frames (ORFs) are indicated by boxes. The numbers inside the boxes are the approximate mol wt in kDa of the proteins encoded by each ORF. The coat protein ORF is denoted by CP. The position of the readthrough termination codon on RNA-1 is indicated by an asterisk. KeywordsCoat ProteinSodium Phosphate BufferTobacco Mosaic VirusTobacco Rattle VirusThioglycollic AcidThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.