Abstract
ABSTRACTPurpose:The rat cervicitis model was established with 20% phenol glue to explore the therapeutic effect of Kangfuxiaomi shuan II on rat cervicitis and its mechanism.Methods:After modeling, the rats were treated with Shuangzuotai suppository (37.84 mg/kg), Kangfuxiaoyan shuan (205.6 mg/kg) and Kangfuxiaomi shuan II (40, 80, 160 mg/kg). The histopathological changes and injury degree of cervix in rats were evaluated by vulvar inflammation score and organ index. The therapeutic effect of Kangfuxiaomi shuan II on cervicitis was evaluated by detecting the levels of copper-protein (CP), C-reactive protein (CRP), Rat interleukin 6 (IL-6), superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and epidermal growth factor (EGF), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in cervical tissue.Results:Compared with the model group, the vulvar inflammation score and cervical index of rats in other groups decreased significantly (P<0.01). Kangfuxiaomi shuan II could significantly reduce the levels of CP, CRP, and MDA in serum of rats with cervicitis, and significantly increase the activity of SOD in serum of rats with cervicitis (P<0.01). The levels of EGF and iNOS in cervical tissue of rats also increased in different degrees, while the level of COX-2 decreased significantly (P<0.01), which significantly improved the pathological degree of vulvar inflammation in rats with cervicitis.Conclusions:Kangfuxiaomi shuan II has a certain therapeutic effect on cervicitis in rats, and its mechanism may be related to the regulation of inflammatory cytokine network and immunity.
Highlights
Cervicitis is a common clinical inflammatory syndrome
Inadequate treatment of acute cervicitis often leads to chronic cervicitis[2], which would gradually develop into cervical erosion, endometritis, or pelvic inflammation, etc., and even adjacent organ will be infected, such as cystitis[3,4]
Kangfuxiaoyan shuan (Kuihua Pharmaceutical Co., Batch number 20131124); shuangzuotai shuan (Hubei Dongxin Pharmaceutical Co., Batch number 20150416); hard fat (AR, Chengdu Lutianhua Kesen Co., Batch number: 141004); kangfuxiaomi shuan II; copper-protein (CP) kit (Batch number 20170328); C-reactive protein (CRP) kit (Batch number 20170714); malondialdehyde (MDA) kit (Batch number 20170628); superoxide dismutase (SOD) kit (Batch number 20170606); inducible nitric oxide synthase kit (Batch number 20171014); epidermal growth factor (EGF) kit (Batch number 20170812); and cyclooxygenase-2 (COX-2) kit (Batch number 20170416) were all provided by Nanjing Jiancheng Bioengineering Institute, likewise Rat interleukin 6 (IL-6) enzyme-linked immunosorbent assay (ELISA) kit (Neobioscience Co., Batch number R170828-002a)
Summary
Cervicitis is a common clinical inflammatory syndrome. The main causes of cervicitis are cervical injury and pathogen infection, and the main pathological changes are inflammation and exudation of the columnar epithelium of the uterine endocervix[1]. Inadequate treatment of acute cervicitis often leads to chronic cervicitis[2], which would gradually develop into cervical erosion, endometritis, or pelvic inflammation, etc., and even adjacent organ will be infected, such as cystitis[3,4]. Western medicine is often used in the clinical treatment of cervicitis. Antimicrobials and hormones work better in the short term, the longterm use of a large number of antibiotics will result in drug resistance, disorder of self-flora, decline of immunity, etc[5,6]. It is necessary to find an appropriate alternative treatment
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