Abstract
This study was based on network pharmacology and molecular docking combined with experiments in vitro to explore the potential anti-inflammatory mechanism of Portulaca oleracea L. flavone (POL-F). First, the core targets of inflammation in purslane were screened through various databases and software, GO and KEGG analysis and molecular docking were performed. Then, mouse macrophage RAW264.7 was selected as the study object, and the safe experimental concentration of POL-F was confirmed by CCK-8 assay. The cells were divided into a control group, an LPS inflammatory cell model group, and an LPS inflammatory cell model group treated with different concentrations of POL-F. The secretion of NO in each group was detected by the Griess method. The expressions of IL-1β, IL-7, and TNF-α in the supernatant were detected by ELISA. The regulatory effects of POL-F on p-PI3K and PAKT signaling pathway proteins in cells of each group were detected by Western blot. The results showed that Pol-F treated with 100, 200, and 400 μg/ mL had no toxic effect on cells. The three concentrations of POL-F showed inhibitory effect on NO secretion of inflammatory cells, and the inhibitory activity was the best at 400 μg/ml. The expression of p-PI3K and PAKT protein was also inhibited after POL-F treatment. In conclusion, POL-F has a significant inhibitory effect on inflammatory response in vitro.
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