Abstract

Objective To investigate the effect of PPP2R5C downregulation by RNA interference using gene chip technology on gene expression related to TNF- related apoptosis- inducing ligand (TRAIL) signaling pathway, basing on the inhibition of proliferation of chronic myelogenous leukemia (CML) cell line K562 by PPP2R5C downregulation showed in previous studies. Methods The PPP2R5C gene in silence K562 cell line by nucleofection was cultured for 48h. The cell RNA was isolated and analyzed by Affymetrix gene microarray after qualified standard control. Genespring GX 11.0 software was used to analyze the differential expression profile of 21 genes related to TRAIL signaling pathway. Results Microarray revealed differential expression among all the genes, including 8 up- regulated genes and 13 down- regulated genes. The significantly up - regulated genes were FASLG, TNFSF11 and RIPK1, and the significantly down - regulated genes were CFLAR, TNFRSF10B, TRAF2, TNFRSF10D, TNFRSF10C, TNF and CASP10. Conclusion Down- regulating PPP2R5C gene in K562 cells relates to the inhibition of TRAIL signaling pathway genes, thus inhibiting the proliferation of K562 cells and promoting the apoptosis of K562 cells. Key words: Apoptosis; Tumor necrosis factors; Gene silencing; PPP2R5C gene

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