TNFR1 and TNFR2 Expression and Induction on Human Treg Cells from Type 1 Diabetic Subjects

Douglas Burger,Georges Tahhan,Danielle Baum,Melanie Heinrich,Denise Faustman,Menghan Zhao,Elise Hsu,Sarah Warden,Limei Wang,Peter Reinhold

doi:10.3390/antib4010034

Douglas Burger, Georges Tahhan + Show 8 more

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https://doi.org/10.3390/antib4010034

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Journal: Antibodies	Publication Date: Mar 3, 2015
Citations: 30	License type: CC BY 4.0

Affiliation: Massachusetts General Hospital, Harvard University

Abstract

Several autoimmune diseases are marked by a deficiency of soluble tumor necrosis factor (TNF). The TNF deficiency is caused in at least one autoimmune disease, multiple sclerosis, by an overabundance of TNF receptor 1 (TNFR1). Excess TNFR1 binds and inactivates TNF and this leaves less TNF bioavailable. This study sought to determine if expression of fresh or IL2-stimulated TNF receptors on Tregs cells, an important immunoregulatory cell involved in autoimmunity, is altered in type I diabetes. Standard fluorescence analysis was used to examine the levels of TNFR1 and TNFR2 on human Tregs in patients with type I diabetes (T1D) or controls. Fresh Tregs from T1D compared to control Tregs had identical levels of TNFR1. In marked contrast, Type 1 diabetic patients Treg cells had statistically elevated levels of TNFR2 compared to controls. Tregs stimulated with IL2 from both T1D and controls showed marked increase of TNFR2 expression in a dose-response manner, but the dose response increase in TNFR2 was significantly higher for T1D Treg cells. No IL2 dose-response was present for TNFR1 on either T1D or control Tregs exposed to IL2. A large study of serum for secreted levels of TNFR2 also revealed elevated circulating levels consistent with the elevated surface expression on Tregs. These findings suggest that abnormal regulation of TNFR2 expression with elevated cellular and secreted levels of TNFR2 is a characteristic of Type 1 diabetes. It is possible that the relative deficiency of TNF in type I diabetes, in contrast to multiple sclerosis, is caused by excess expression of TNFR such as TNFR2, a binding structure for inactivating TNF.

Highlights

Tumor necrosis factor (TNF) is the signature protein for the innate immune response
We looked at freshly isolated Tregs from diabetic and control subjects for unstimulated basal levels of TNF receptor 1 (TNFR1) and TNFR2
Some forms of human autoimmunity, including type 1 diabetes, are characterized by a paucity of functional TNF, an important cytokine for both Treg survival and proper negative selection of autoreactive CD8 T cells. It is known for some autoimmune diseases, like multiple sclerosis, dysregulation of TNF receptors, such as TNFR1 over-secretion, contributes to low levels of biologically active TNF [22,32]

Summary

Introduction

Tumor necrosis factor (TNF) is the signature protein for the innate immune response. In response to microorganism invasion, the mammalian host immediately secretes TNF to kill or inactivate the microorganism. In addition to infection control, TNF, in the host, is important for many beneficial immune effects. TNF is obligatory for survival of regulatory T cells (Tregs) and is able to kill autoreactive. T cells (in the periphery) [1]. Deficiencies of TNF may drive diverse autoimmune diseases [2,3]. Supporting Evidence That TNF is Deficient in Many Autoimmune Diseases

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