TNF-alpha, IL-4 and IL-10 cytokines induces changes in HO-1 and HDC expression in cervical cancer cells

Abstract

Event Abstract Back to Event TNF-alpha, IL-4 and IL-10 cytokines induces changes in HO-1 and HDC expression in cervical cancer cells Paulina Gomez-Lomeli1, Pablo C. Ortiz-Lazareno1*, Alejandro Bravo-Cuellar1, Georgina Hernandez-Flores1, Jorge R. Dominguez-Rodriguez1, Luis F. Jave-Suarez1 and Adriana Aguilar-Lemarroy1 1 Instituto Mexicano del Seguro Social, Mexico Background Chronic infection with human papilloma virus (HPV) has been associated as an important factor in generating cancer. E6 and E7 viral proteins are involved in cell transformation. Likewise tumor cells generate escape mechanisms as the expression of molecules that regulate the immune response. HDC and HO-1 exert anti-apoptotic functions. These enzymes may represent another mechanism of resistance in cervical cancer cells. Objectives. We analyzed the gene and protein expression of heme oxygenase (HO-1) and histidine decarboxylase (HDC) enzymes in HPV+ HeLa and SiHa cervical cancer cell lines and HPV- C33A cancer cells treated with TNF-α, I-4 and IL-10 cytokines. Methods HeLa, SiHa and C33A cervical cancer cells were treated with pro and anti-inflammatory cytokines; mRNA was extracted and analyzed expression of HO1 and HDC by real-time PCR. Protein expression was assessed by flow cytometry. Results Cytokines treatment induces a downregulation in HDC gene expression in HPV+ cancer cells in comparison with untreated tumor cells. In HeLa and SiHa cells this gene is downregulated whereas in C33A cancer cells we observed upregulation of HDC gene. In general HDC and HO-1 protein expression was reduced in cancer cells treated with pro and anti-inflammatory cytokines. Conclusions HDC and HO-1 protein expression is modified when the cells were treated with cytokines. In HeLa, SiHa and C33 cancer cells the expression of these enzymes is similar, however at the gene level the behavior is different between cancer cell lines, and this could be due to the presence of HPV infection in some cancer cells. References Moody, C. A. and L. A. Laimins (2010). "Human papillomavirus oncoproteins: pathways to transformation." Nat Rev Cancer 10(8): 550-560. Kareva, I. and P. Hahnfeldt (2013). "The Emerging "Hallmarks" of Metabolic Reprogramming and Immune Evasion: Distinct or Linked?" Cancer Res Gozzelino, R., et al. (2010). "Mechanisms of Cell Protection by Heme Oxygenase-1." Annual Review of Pharmacology and Toxicology 50(1): 323-354. Ichikawa, A., et al. (2010). "Molecular biology of histidine decarboxylase and prostaglandin receptors." Proceedings of the Japan Academy, Series B 86(8): 848-866. Keywords: Heme-oxynenase 1, Histidine Decarboxylase, Cervical cancer cells, tNF-alpha, Anti-inflammatory cytokines Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Gomez-Lomeli P, Ortiz-Lazareno PC, Bravo-Cuellar A, Hernandez-Flores G, Dominguez-Rodriguez JR, Jave-Suarez LF and Aguilar-Lemarroy A (2013). TNF-alpha, IL-4 and IL-10 cytokines induces changes in HO-1 and HDC expression in cervical cancer cells. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00634 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Pablo C Ortiz-Lazareno, Instituto Mexicano del Seguro Social, Guadalajara, Mexico, pablolazareno@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Paulina Gomez-Lomeli Pablo C Ortiz-Lazareno Alejandro Bravo-Cuellar Georgina Hernandez-Flores Jorge R Dominguez-Rodriguez Luis F Jave-Suarez Adriana Aguilar-Lemarroy Google Paulina Gomez-Lomeli Pablo C Ortiz-Lazareno Alejandro Bravo-Cuellar Georgina Hernandez-Flores Jorge R Dominguez-Rodriguez Luis F Jave-Suarez Adriana Aguilar-Lemarroy Google Scholar Paulina Gomez-Lomeli Pablo C Ortiz-Lazareno Alejandro Bravo-Cuellar Georgina Hernandez-Flores Jorge R Dominguez-Rodriguez Luis F Jave-Suarez Adriana Aguilar-Lemarroy PubMed Paulina Gomez-Lomeli Pablo C Ortiz-Lazareno Alejandro Bravo-Cuellar Georgina Hernandez-Flores Jorge R Dominguez-Rodriguez Luis F Jave-Suarez Adriana Aguilar-Lemarroy Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.