Abstract
Abstract Infection induced immune responses cause extensive bone destruction in periodontitis. We previously reported that TNF-alpha converting enzyme (TACE) play a role in soluble RANKL and soluble TNF-alpha cleavage from activated lymphocytes in periodontitis, and these soluble osteoclastogenic cytokines induce osteoclast activation from distant site. Interferon-gamma is known as anti-osteoclastogenic cytokine produced from activated T cells, but little is known about the post-transcriptive modification, especially in inflamed tissue. We hypothesized that TACE might modify interferon-gamma and attenuate anti-osteoclastogenic activity. Recombinant interferon-gamma is incubated with intact or heat-inactivated recombinant TACE, electrophoresed in SDS-PAGE gel, and silver stain was done. In addition, the interferon-gamma concentrations of above samples are measured with ELISA. Recombinant TACE is added into the supernatant of activated mouse splenocyte, and measured interferon-gamma concentration. Silver stain revealed that not heat-inactivated but intact recombinant TACE reduced the 16 kDa band of recombinant interferon-gamma. ELISA also showed the reduction of interferon-gamma concentration by the incubation with recombinant TACE. These data suggest that, TACE might degradate interferon-gamma and attenuate anti-osteoclastogenic activity of interferon-gamma produced from activated T cells in periodontitis.
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