Tần suất và đột biến gen LMP1 của virus Epstein-Barr ở mẫu sinh thiết vòm của bệnh nhân ung thư vòm mũi họng điều trị tại Bệnh viện Ung bướu Cần Thơ

Abstract

Latent Membrane Protein 1 (LMP1) gen of Epstein-Barr virus (EBV) (LMP1 EBV) and the mutation in LMP1 EBV were important for the formation of malignant tumors in nasopharyngeal carcinoma (NPC) in patients with EBV infection. The objective of the study was to determine the frequency and loss 30 bp mutation of the LMP1 gene in the biopsy specimen of the NPC patient. A cross sectional descriptive study was carried out of 65 biopsy specimes of patients, who was confirmed as nasopharynreal carcinoma at the Can Tho Oncology Hospital. Polymerase Chain Reaction (PCR) with LMP1 primer (168373-168174) was used to determine the frequency of LMP1 EBV gene and sequening technique was applied to identify LMP1 gene mutation. As a result, the rate of LMP1 EBV in nasopharyx biopsy specimens was 61.5% (40/65). Besides, there was 57.5% (23/40) of 30 bp loss mutant on LMP1 EBV gene was indentified among of LMP1 EBV in nasopharyx biopsy specimens. In conclusions, the rate of LMP1 EBV on biopsy specimens of NPC in Can Tho was 61.5% with the common mutation pattern of 30bp loss.