Abstract
A series of mutant terminal inverted repeats (IRs), having2 bp subtitutions at various sites within the 38-bp IR sequence of the ampicillin-resistance transposon Tn 3, were tested for transposition immunity to Tn 3. Mutations within region 1–10 in the IR did not affect transposition immunity, while mutations within region 13–38 inactivated the immunity function. These two regions corresponded to domain A which was not bound specifically by Tn 3 transposase and to domain B which was bound by the transposase, respectively. This indicates that specific binding of transposase to domain B within the IR sequence is responsible for transposition immunity.
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