Abstract
BackgroundIntramuscular adipocytes differentiation is a complex process, which is regulated by various transcription factor, protein factor regulators and signal transduction pathways. However, the proteins and signal pathways that regulates goat intramuscular adipocytes differentiation remains unclear.ResultIn this study, based on nanoscale liquid chromatography mass spectrometry analysis (LC-MS/MS), the tandem mass tag (TMT) labeling analysis was used to investigate the differentially abundant proteins (DAPs) related with the differentiation process of goat intramuscular adipocytes. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes enrichment and protein-protein interaction network analyses were performed for the characterization of the identified DAPs. The candidate proteins were verified by parallel reaction monitoring analysis. As a result, a total of 123 proteins, 70 upregulation proteins and 53 downregulation proteins, were identified as DAPs which may be related with the differentiation process of goat intramuscular adipocytes. Furthermore, the cholesterol metabolism pathway, glucagon signaling pathway and glycolysis / gluconeogenesis pathway were noticed that may be the important signal pathways for goat Intramuscular adipocytes differentiation.ConclusionsBy proteomic comparison between goat intramuscular preadipocytes (P_IMA) and intramuscular adipocytes (IMA), we identified a series protein that might play important role in the goat intramuscular fat differentiation, such as SRSF10, CSRP3, APOH, PPP3R1, CRTC2, FOS, SERPINE1 and AIF1L, could serve as candidates for further elucidate the molecular mechanism of IMF differentiation in goats.
Highlights
Intramuscular adipocytes differentiation is a complex process, which is regulated by various transcription factor, protein factor regulators and signal transduction pathways
By proteomic comparison between goat intramuscular preadipocytes (P_IMA) and intramuscular adipocytes (IMA), we identified a series protein that might play important role in the goat intramuscular fat differentiation, such as serine and arginine rich splicing factor 10 (SRSF10), cysteine and glycine rich protein 3 (CSRP3), APOH, PPP3R1, CRTC2, FOS, SERPINE1 and Allograft inflammatory factor 1 like (AIF1L), could serve as candidates for further elucidate the molecular mechanism of Intramuscular fat (IMF) differentiation in goats
From the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, we found two pathways may involve in regulating adipocyte differentiation, that were cholesterol metabolism signaling pathway and glucagon signaling pathway
Summary
Intramuscular adipocytes differentiation is a complex process, which is regulated by various transcription factor, protein factor regulators and signal transduction pathways. The proteins and signal pathways that regulates goat intramuscular adipocytes differentiation remains unclear. Intramuscular fat (IMF) is the adipose tissue between muscle fibers, known as marbling. Adipose tissue is mainly composed of a large number of adipocytes, which are generated through the proliferation and differentiation of preadipocytes. The differentiation of preadipocytes into adipocytes is a complex process, which is regulated by various transcription factor, protein factor regulators and signal transduction pathway [6,7,8]. MiR-429 inhibits subcutaneous and intramuscular preadipocytes differentiation while promotes proliferation by directly binding to the 3′-UTRs of KLF9 and p27 [9]. Using HPLCtandem mass spectrometry and methylated RNA immunoprecipitation (meRIP), found that MTCH2 promotes adipogenesis in pig P_IMA via an m6A-YTHDF1dependent mechanism [10]
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