Abstract
BackgroundThyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulatory mechanism of TMPO-AS1 in TC.MethodsTMPO-AS1 and TMPO expression in TC tumors and cells was detected by TCGA database and QRT-PCR assay respectively. CCK-8, EDU, TUNEL and western blot assays were conducted to identify the biological functions of TMPO-AS1 in TC. Luciferase reporter and RNA pull down assays were conducted to measure the interaction among TMPO-AS1, TMPO and miR-498.ResultsTMPO-AS1 was overexpressed in TC tissues and cell lines. Knockdown of TMPO-AS1 suppressed cell growth and accelerated cell apoptosis in TC. Furthermore, downregulation of TMPO-AS1 suppressed TMPO expression in TC. The data suggested that TMPO expression was upregulated in TC tissues and cell lines and was positively correlated with TMPO-AS1 expression in TC. Furthermore, the expression of miR-498 presented low expression in TC cells. And miR-498 expression was negatively regulated by TMPO-AS1, meanwhile, TMPO expression was negatively regulated by miR-498 in TC cells. Besides, it was confirmed that TMPO-AS1 could bind with miR-498 and TMPO in TC cells. In addition, it was validated that TMPO-AS1 elevated the levels of TMPO via sponging miR-498 in TC cells.ConclusionsTMPO-AS1 promotes cell proliferation in TC via sponging miR-498 to modulate TMPO.
Highlights
Thyroid cancer (TC) is the most frequent endocrine malignancy
The expression of TMPO‐AS1 is significantly upregulated in TC tissues and cells and facilitates the functions of TC cells At first, we obtained the information from GEPIA database and found that TMPO-AS1 was upregulated in TC tissues (Fig. 1a, p < 0.01)
The level of TMPO-AS1 was evaluated in four TC cells and human normal thyroid cell line nthy-ori3-1
Summary
Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulatory mechanism of TMPO-AS1 in TC. Thyroid cancer (TC) is a typical subtype of endocrine malignancy. Long non-coding RNAs (lncRNAs) are a group of noncoding RNAs longer than 200 nucleotides [6, 7]. Previous literature has verified that lncRNAs exerted key roles in the progression of multiple cancers and worked as either oncogenes or tumor suppressors. LncRNA STCAT16 suppresses cell growth in gastric cancer [8]. LncRNA PEG10 sponges miR-134 to exert its oncogenic function in bladder cancer [9].
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