Abstract
ObjectiveTo investigate the correlation between the single nucleotide polymorphisms (SNPs) in the toll-like receptor 4 (TLR4) gene and the susceptibility to chronic periodontitis.Design241 Chinese subjects from the cohort of Beijing Shijingshan Community were recruited. Buccal swab samples, the whole unstimulated saliva and periodontal clinical parameters were collected. Human DNA extracted from buccal swab samples were used for genotyping eight SNPs of the TLR4 gene (rs11536889, rs1927906, rs1927911, rs2149356, rs4986790, rs4986791, rs2737190, rs787384) by the Sequenom MassARRAY system. Porphyromonas gingivalis (P. gingivalis) was detected from the deposition of the whole unstimulated saliva through polymerase chain reaction (PCR) method based on 16S rRNA. The correlation between SNPs of TLR4 and chronic periodontitis susceptibility in the whole subjects and the patients detected with P. gingivalis was investigated.ResultsThe variants of rs4986790 and rs4986791 were not found in 241 Chinese subjects. Moreover, there was no significant difference in the distribution of theother6 SNPs of TLR4 between groups of none/mild -periodontitis and moderate/severe-periodontitis subjects. When combined with P. gingivalis infection, rs1927911 (TT/CC+CT), rs2149356 (TT/GG+GT) and rs2737190 (GG/AA+AG) were independent risk factors of chronic periodontitis.ConclusionThree SNPs of TLR4, i.e., rs1927911 (TT/CC+CT), rs2149356 (TT/GG+GT) and rs2737190 (GG/AA+AG), were associated with moderate/severe chronic periodontitis in Chinese population infected with P. gingivalis. P. gingivalis, which interacted with TLR4 gene plays an important role in the pathogenesis of periodontitis.
Highlights
Periodontal diseases are initiated by microorganisms in the subgingival biofilm, of which Porphyromonas gingivalis (P. gingivalis, Pg) was revealed to be the most prevalent gramnegative bacteria among patients with chronic periodontitis when compared with healthyHow to cite this article Li W, Cao X, He L, Meng H, Yang B, Liao Y. 2019
It is shown that the Moderate/severe chronic periodontitis (msCP) patients had a higher mean age and percentage of smoking than that of the none/mild chronic periodontitis (nmCP) patients, both with statistical significance
Though the msCP group had a higher percentage of diabetes, the percentage of diabetes and hypertension showed no significant difference between two groups
Summary
Periodontal diseases are initiated by microorganisms in the subgingival biofilm, of which Porphyromonas gingivalis (P. gingivalis, Pg) was revealed to be the most prevalent gramnegative bacteria among patients with chronic periodontitis when compared with healthyHow to cite this article Li W, Cao X, He L, Meng H, Yang B, Liao Y. 2019. Periodontal diseases are initiated by microorganisms in the subgingival biofilm, of which Porphyromonas gingivalis (P. gingivalis, Pg) was revealed to be the most prevalent gramnegative bacteria among patients with chronic periodontitis when compared with healthy. TLR4 polymorphisms may increase susceptibility to periodontitis in Pg-positive individuals. P. gingivalis mainly colonizes at subgingival sites, especially in deep pockets, of patients with periodontitis (Farias et al, 2012). Its prevalence was much higher and its amount was far higher in moderate and severe periodontitis patients than the mild periodontitis patients or periodontally healthy subjects (Feng et al, 2009). Several studies showed that detection of P. gingivalis in saliva samples matched with that in subgingival samples of periodontitis patients (Feng et al, 2009; He et al, 2012)
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