TLR4 polymorphism and haplotype are associated with obesity and lipid profile in young population: a pilot study.

Abstract

The single nucleotide polymorphisms in the TLR4 gene can decrease or increase the response to lipopolysaccharide, increasing the susceptibility to inflammatory diseases, affecting the expression or receptor function by inducing a low-grade chronic inflammatory response. The objective of this study was to evaluate the association of SNPs -2570 A > G (rs2737190), -2081 G > A (rs10983755), 896 A > G (rs 4986790), and 1196 C > T (rs4986791) of the TLR4 gene with obesity and metabolic alterations in the young population. In this study, it was found that the carriers of the heterozygous genotype of the SNPs -2081 G > A, 896 A > G, and 1196 C > T confer a higher risk of developing obesity (OR=3.73, p=0.018; OR=5.66, p=0.014, and OR=8.95, p=0.014, respectively). Also, with the lipid profile, the SNP -2081 G > A was associated with total cholesterol (TC)≥ 200mg/dL (OR=3.91, p=0.020) and Kannel index >3% (OR=4.00, p=0.008). The SNP 896 A>G was associated with LDL-c ≥100 mg/dL (OR=3.64, p=0.040) and Kannel index >3% (OR=4.33, p=0.016), and the SNP 1196 C>T was associated with TC≥200mg/dL (OR=4.37, p=0.048), Castelli index >4.5/>5% (OR=5.33, p=0.016), and Kannel index >3% (OR=16.00, p=0.001). Finally, the AGGT haplotype was associated with Castelli index >4.5/>5% (OR=5.40, p=0.015) and Kannel index >3% (OR=10.46, p<0.001), and the AAAC haplotype was associated with obesity (OR=3.56, p=0.020), TC≥200mg/dL (OR=4.04, p=0.007), LDL-c≥100mg/dL (OR=2.98, p=0.030) and Kannel index >3% (OR=4.20, p=0.002). The heterozygous genotype of the SNPs -2081 G > A, 896 A > G and 1196 C > T of the TLR4 gene was associated with altered lipid profile and development of obesity in young university students of Guerrero State, Mexico.