Abstract
TLC with a stationary-phase gradient has been used for purification and quantitative analysis of taxol in the needles and stems of female and male specimens of Taxus baccata L. harvested during the vegetative period of the plant. Parts of different TLC plates were connected by use of a Migas device. Separated taxol-containing zones were moved 1 cm, with methanol, on to another plate. The lipophilic substance zone was cut out after separation of the sample on a TLC Si60 F 254s plate with n -heptane-ethyl acetate 5:5 ( v/v ) as mobile phase. Further separation of taxol from accompanying hydrophilic substances was carried out on RP18W F 254s HPTLC plates with methanol-water 8:2 ( v/v ) as mobile phase. The taxol-containing fraction was finally separated on Si60 F 254s TLC plates with chloroform-acetone 15:5 ( v/v ) as mobile phase. The optimized method for taxol separation was used for quantitative densitometric analysis. The results revealed differences in the amounts of taxol in the needles and stems of Ta...
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