Abstract

A chromatographic-densitometric method was developed for determination of oxyhyphenonium bromide and its degradation products under UV/VIS spectra. Silica gel TLCF254 plates were used as stationary phase and butanol-1–glacial acetic acid–water (12.8:2:2.8 v/v/v) as mobile phase. Densitometric measurements were done at λ ≈ 225 nm and λ ≈ 530 nm. Degradation products α-cyclohexyl-α-hydroxybenzeneacetic acid and N,N-diethyl-N-methylaminethanol were determined by 1HNMR method. The chromatographic-densitometric method is specific for studied compounds. Besides, it is shown that this method is very sensitive, precise, and has a broad range of linearity.

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