Abstract
Interest in the antioxidant activity of bamboo leaves is growing. To discover new sources of natural antioxidants, a TLC bioautography method combined with a new image processing method was developed to evaluate the antioxidant activity of leaf extracts from 15 different species of bamboo. The results showed that the methanolic extract of Bambusa. textilis McClure possessed the highest antioxidant activity among the selected bamboo species. To rapidly identify the antioxidant compounds, the crude extract of B. textilis McClure was analysed by HPLC-UV, and HPLC-micro-fractionation of the extract was carried out. Based on TLC bioautography-guided fractionation, three antioxidant fractions were isolated from B. textilis McClure by preparative chromatography. These three antioxidant compounds were identified as isoorientin 4''-O-β-D-xylopyranoside (1), isoorientin 2''-O-α-L-rhamnoside (2) and isoorientin (3) according to their UV, MS, and NMR data. The proposed TLC screening method could therefore be an easy way to evaluate the antioxidant activity of bamboo leaves, and the results achieved should prove very helpful for promoting their utilization, as B. textilis McClure can be considered a promising plant source of natural antioxidants.
Highlights
There are more than 1250 bamboo (Graminaceae) species distributed all over the World
The proposed thin-layer chromatography (TLC) screening method could be an easy way to evaluate the antioxidant activity of bamboo leaves, and the results achieved should prove very helpful for promoting their utilization, as B. textilis McClure can be considered a promising plant source of natural antioxidants
We evaluated the antioxidant activities of 15 different species of bamboo using a TLC
Summary
There are more than 1250 bamboo (Graminaceae) species distributed all over the World. Several studies have been carried out to identify the antioxidants from different species of bamboo, such as Phyllostachys edulis [1], Fargesia robusta [3], and Sasa borealis [6]. The antioxidative compounds from different species of bamboo could be different. Some strategies have to be designed for the screening and identification of antioxidative compounds from bamboo leaves. B. textilis McClure is a kind of bamboo species used in traditional medicine in China. To the best of our knowledge, the active antioxidant compounds of B. textilis McClure have not been identified. We evaluated the antioxidant activities of 15 different species of bamboo using a TLC bioautography assay. Textilis McClure showed the strongest antioxidant activity among the selected bamboo species. The main antioxidant compounds of Bambusa. textilis McClure were isolated and identified based on preparative-HPLC, MS, and NMR techniques
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