Abstract
Conditions have been established for TLC analysis and determination of the flavonol truxinic esters daglesiosides I and II in species of the family Pinaceae . Optimization of the separation of eleven flavonoids, including daglesiosides I, II, III, and IV, trans -tiliroside, trans -ditiliroside, kaempferol, quercetin, isorhamnetin, hyperoside, and astragalin, which are present in the methanol extract of Pseudotsuga menziesii , was performed on two types of layer — glass plates coated with RP-18F 254S and diol F 254S . Good resolution was achieved on diol F 254S layers by use of multiple gradient development with a mixture of chloroform and ethyl acetate. Under these conditions the presence of daglesioside I was demonstrated in two other species of Pinaceae — Cedrus atlantica and Cedrus atlantica var. glauca — and the flavonoid complex occurring in Pseudotsuga menziesii was completely separated by use of 2D TLC on coupled silica gel and RP-18 layers.
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