Abstract
This research article describes a simple and sensitive thin-layer chromatographic (TLC)-densitometric method for simultaneous determination of guaifenesin (GUF) and pseudoephedrine HCl (PSH) in their binary mixtures and in the presence of guaifenesin impurity and related substance, guaiacol. The proposed chromatographic method has been developed using silica gel plates 60 F254 as a stationary phase with hexane-acetone-ethyl acetate-triethylamine-water (3:4:3:0.1:0.3, by volume) as a developing system followed by densitometric measurements at 208 (for pseudoephedrine HCl and guaifenesin) and 278 nm (for guaiacol). Experimental conditions have been optimized to get the desired separation and sensitivity. Calibration curves for the three studied components were constructed using polynomial equation which was superior to linear equation with respect to correlation coefficients and quantified ranges (14–25, 2–12, and 0.1–1.1 μg band−1) for pseudoephedrine HCl, guaifenesin and guaiacol, respectively. The develo...
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