TLC 상 유도체화 반응을 이용한 아민 계 화합물의 Screening 방법

Abstract

Abstract: Methamphetamine is an amine-containing illegal drug and is distributed unlawfully in South Korea.Finding a rapid, convenient and semi-quantitative determination method for methamphetamine is a very importantissue in the area of forensic drug testing. As an effort to develop new screening method, the reactions betweenthree organic compounds which are structurally similar to methamphetamine and N-(9-fluorenylmethoxycarbonyloxy)succinimide (FMOC-NHS) were performed on silica gel (SiO 2 ) TLC plates. Three reference compounds weresynthesized and used for the identification, comparison and study of the limit of detection (LOD) of the productsobtained from a direct reaction on a TLC plate. As a result, FMOC-NHS as a derivatization reagent generatedcompounds containing highly UV-active functional groups on the TLC plate after reacting with primary- andsecondary amines. In the experiment 2D the LOD of amines was in the range of 0.045 and 0.01 mg/mL (2 µL/spot), and in 1D the LOD was in the range of 0.002 and 0.007 mg/mL (2 µL/spot). The LODs of the compoundstested were dependent on the concentration of the derivatizing reagent. 요약: 국내에서 불법 유통 되거나 남용되고 있는 methamphetamine은 아민을 함유한 불법약물로서 그에 대한 신속하고 용이한 동정 및 정량적인 분석 방법은 불법약물 검사의 중요한 이슈가 되어왔다. 새로운 screening방법을 모색하기 위해 methamphetamine과 구조적으로 유사한 3종의 유기 화합물을 silica gel(SiO