Abstract
The purpose of this investigation was to develop a rapid, simple titrimetric method which could be used to replace the more expensive and time consuming Warburg method for the assay of milk arylesterase (A-esterase) activity. A titrimetric method was selected on the basis of preliminary experimentation. Optimum conditions (e.g., temp, pH, substrate concentration, and time) for the assay of milk A-esterase activity were then investigated. On the basis of these results a standard titrimetric method was suggested. This procedure was then compared with the Warburg method (r = 0.97, n = 182). The coefficients of variation between duplicate samples were 2.6 and 5.2%, respectively, for the Warburg and titrimetric methods. The precision of the titrimetric procedure was increased (approximately fourfold) by titrating to a constant pH, using a potentiometer instead of phenolphthalein.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
