Titanium Functionalized with Polylysine Homopolymers: In Vitro Enhancement of Cells Growth.

Maria Contaldo,Kenan Ferati,Alfredo De Rosa,Davide Malacrinò,Andrea Ballini,Marina Di Domenico,Antonia Feola,Marcella La Noce,Edit Xhajanka,Arberesha Bexheti-Ferati,Ludovica Nucci,Francesco Inchingolo

doi:10.3390/ma14133735

Abstract

In oral implantology, the success and persistence of dental implants over time are guaranteed by the bone formation around the implant fixture and by the integrity of the peri-implant mucosa seal, which adheres to the abutment and becomes a barrier that hinders bacterial penetration and colonization close to the outer parts of the implant. Research is constantly engaged in looking for substances to coat the titanium surface that guarantees the formation and persistence of the peri-implant bone, as well as the integrity of the mucous perimeter surrounding the implant crown. The present study aimed to evaluate in vitro the effects of a titanium surface coated with polylysine homopolymers on the cell growth of dental pulp stem cells and keratinocytes to establish the potential clinical application. The results reported an increase in cell growth for both cellular types cultured with polylysine-coated titanium compared to cultures without titanium and those without coating. These preliminary data suggest the usefulness of polylysine coating not only for enhancing osteoinduction but also to speed the post-surgery mucosal healings, guarantee appropriate peri-implant epithelial seals, and protect the fixture against bacterial penetration, which is responsible for compromising the implant survival.

Highlights

IntroductionDental implants are multi-material prostheses that replace tooth roots with screwlike metal fixtures surgically inserted into the edentulous bone that are connected by the creativecommons.org/licenses/by/ 4.0/)
Cell growth analysis and viability staining with trypan blue showed that human dental pulp stem cells (hDPSCs)
Cell (p growth viability staining with trypan showed noticeably increased (p cultured in standard condition and on titanium with and without poly-L-lysine showed the same trend in growth; while the titanium alone slightly negatively affects the viability for cells (p < 0.01), the cell growth on the poly-L-lysine coated titanium was noticeably increased (p < 0.001) (Figure 4)

Summary

Introduction

Dental implants are multi-material prostheses that replace tooth roots with screwlike metal fixtures surgically inserted into the edentulous bone that are connected by the creativecommons.org/licenses/by/ 4.0/). Dental implants are multi-material prostheses that replace tooth roots with screw-like metal fixtures surgically inserted into the edentulous bone that are connected by the abutment with with an artificial crowncrown that replaces the missing tooth, looking acting identical abutment an artificial that replaces the missing tooth, and looking and acting to the realtoone identical the(Figure real one). A dental implant (a) (a) andand the the oraloral structures (peri-implant mucosa andand peri-implant bone) surrounding the mucosa peri-implant bone) surrounding thefixture fixture(b),. (b),surgically surgicallyinserted inserted in in the the bone.

Methods

Results

Discussion

Conclusion