Titania Nanosheet as a Matrix for Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis and Imaging.

Abstract

Surface-assisted laser desorption/ionization (SALDI) acts as a soft desorption/ionization technique, which has been widely recognized in small-molecule analysis owing to eliminating the requirement of the organic matrix. Herein, titania nanosheets (TiO2 NSs) were applied as novel substrates for simultaneous analysis and imaging of low-mass molecules and lipid species. A wide variety of representative analytes containing amino acids, bases, drugs, peptides, endogenous small molecules, and saccharide-spiked urine were examined by the TiO2 NS-assisted LDI mass spectrometry (MS). Compared with conventional organic matrices and substrates [Ag nanoparticles (NPs), Au NPs, carbon nanotubes, carbon NPs, CeO2 microparticles, and P25 TiO2], the TiO2 NS-assisted LDI MS method shows higher sensitivity and less spectral interference. Repeatability was evaluated with batch-to-batch relative standard deviations for 5-hydroxytryptophan, glucose-spiked urine, and glucose with addition of internal standard, which were 17.4, 14.9, and 2.8%, respectively. The TiO2 NS-assisted LDI MS method also allows the determination of blood glucose levels in mouse serum with a linear range of 0.5-10 mM. Owing to the nanoscale size and uniform deposition of the TiO2 NS matrix, spatial distributions of 16 endogenous small molecules and 16 lipid species from the horizontal section of the mouse brain tissue can be visualized at a 50 μm spatial resolution. These successful applications confirm that the TiO2-assisted LDI MS method has promising prospects in the field of life science.