Abstract
Background. Proteoglycans (PGs) are complex glycosylated molecules playing an important role in cell-cell and cell-matrix interactions and signaling. Expression of PGs and their expression pattern change considerably during malignant transformation of mammalian cells and tissues. Objective. The aim of our work was to investigate tissue-specificity of main PGs expression (glypican-1, perlecan, syndecan-1, aggrecan, versican, CSPG4/NG2, brevican, decorin, lumican) in normal cells (fibroblasts and normal epithelial prostate cells PNT2) and in different human cancer cell lines (prostate, breast, lung, brain, kidney). Expression patterns of main PGs were determined in these cells using reverse transcription polymerase chain reaction analysis and immunocytochemical staining. Results. It was shown that fibroblasts actively expressed PGs, and PNT2 cells had lower (5–6-fold) expression levels of a limited set of PG. In different cancer cell lines, overall transcriptional activities of PGs varied up to 10-fold, although their expression patterns had tissue-specific properties (for example, expression of syndecan-1 is more specific for prostate cancer cells, while perlecan is typical for lung cancer cell lines). Conclusions. Along with this, variability of the PG expression patterns in cell lines of the same tissue of origin was shown, suggesting a possible contribution of the variable PGs expression to intratumoural heterogeneity of cancer cells and their potential as perspective biomarker (s) for personalised cancer diagnostics.
Highlights
Proteoglycans (PGs) are complex glycosylated molecules playing an important role in cell-cell and cell-matrix interactions and signaling
Expression patterns of main PGs were determined in these cells using reverse transcription polymerase chain reaction analysis and immunocytochemical staining
It was shown that fibroblasts actively expressed PGs, and PNT2 cells had lower (5–6-fold) expression levels of a limited set of PG
Summary
Григорьева1, 2 1ФГБНУ «Научно-исследовательский институт молекулярной биологии и биофизики»; Россия, 630117, Новосибирск, ул. 2/12; 2ФГАОУ ВО «Новосибирский национальный исследовательский государственный университет»; Россия, 630090, Новосибирск, ул. Цель работы – изучить тканеспецифичность экспрессии основных ПГ (глипикана-1, перлекана, синдекана-1, аггрекана, версикана, ХСПГ4/NG2, бревикана, декорина и люмикана) в нормальных (фибробласты, эпителиальные клетки предстательной железы PNT2) и опухолевых клеточных линиях человека (рак предстательной железы, молочной железы, легкого, мозга, почки) методом полимеразной цепной реакции c обратной транскрипцией. Опухолевые клеточные линии отличаются по общей транскрипционной активности ПГ (до 10 раз) и паттерну экспрессируемых ПГ, обладая при этом тканеспецифичными признаками (например, экспрессия синдекана-1 более характерна для опухолей предстательной железы, а экспрессия перлекана – для рака легкого). Ключевые слова: внеклеточный матрикс, микроокружение опухоли, протеогликаны, паттерн экспрессии, тканеспецифичность, рак легкого, рак молочной железы, рак предстательной железы
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