Abstract
Native polyacrylamide gel electrophoresis have been used to analyze malate dehydrogenase (MDH), acid phosphatase (Acph) and peroxidase (Px) isoenzymes in different tissues (liver, kidney, muscle and heart) of the tilapia fish, Oreo- chromis niloticus in order to study the tissue specificity of these isoenzymes. Three, two and one fractions have been recorded respectively for the three isoenzymes in different studied tissues. The MDH-1 and MDH-2 have been ex- pressed only in muscle and heart while MDH-3 has been expressed in all studied tissues. The percentage amount of MDH in general varied significantly between muscle and different studied tissues. With respect to acid phosphatase, the percentage amount of the total enzyme showed significant difference between liver and muscle and that this variation may be due to higher gene activity in liver. Peroxidase isoenzyme was recorded in liver and heart only with significant increase in liver. The kidney was the least among the studied tissues in showing gene expression for the studied isoenzymes and therefore, liver, heart and muscle tissues are better applicable in studying the isoenzymatic profiles for fish physiology and systematics.
Highlights
Tilapiini is a highly diverse tribe with more than 70 species belonging to the order Perciformes, family Cichlidae [1] to which the commonly called tilapia belongs
The kidney was the least among the studied tissues in showing gene expression for the studied isoenzymes and liver, heart and muscle tissues are better applicable in studying the isoenzymatic profiles for fish physiology and systematics
The present study aimed to investigate whether the studied isoenzymes differently expressed in the different tissues of the Nile tilapia Oreochromis niloticus cultivated in the Saudi Arabian farms
Summary
Tilapiini is a highly diverse tribe with more than 70 species belonging to the order Perciformes, family Cichlidae [1] to which the commonly called tilapia belongs. LDH and MDH isoenzymes are major system found in fishes. They are classified in different groups on the basis of their possession in different tissues or cell organelles [14,15]. Peroxidase enzymes are involved in phagocytosis and immune cell function [19,20], cell adhesion [21], antioxidant function [22,23] and the oxidative polymerization of hydroquinones to melanin [24]. The present study aimed to investigate whether the studied isoenzymes differently expressed in the different tissues of the Nile tilapia Oreochromis niloticus cultivated in the Saudi Arabian farms
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