Abstract
Serum, urine and tissue from a rat model of chronic kidney disease (CKD) were analysed using nuclear magnetic resonance (NMR) spectroscopy-based metabolomics methods, and compared with samples from sham operated rats. Both urine and serum were sampled at multiple timepoints, and the results have been reported elsewhere (https://doi.org/10.1007/s11306-019-1569-3[1]). The data could be useful to researchers working with human CKD or rat models of the disease. In addition, several different types of NMR spectra were recorded, including 1D NOESY, CPMG, and 2D J-resolved spectra, and the data could be useful for method comparison and algorithm development, both in terms of NMR spectroscopy and multivariate analysis.
Highlights
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Several different types of nuclear magnetic resonance (NMR) spectra were recorded, including 1D nuclear Overhauser effect spectroscopy (NOESY), CPMG, and 2D J-resolved spectra, and the data could be useful for method comparison and algorithm development, both in terms of NMR spectroscopy and multivariate analysis
Biochemistry NMR-based metabolomics in an animal model of chronic kidney disease 1 H NMR spectra Bruker 600 MHz Avance DRX-600 NMR spectrometer equipped with a TXI probe Raw and processed 1H NMR spectra The 5/6 nephrectomy rat model of chronic kidney disease (CKD) was compared to sham operation, by analysing NMR spectra of urine, serum and tissue samples
Summary
The data consists of raw and processed proton nuclear magnetic resonance (1H NMR) spectra, which are in standard Bruker format. NMR spectra were acquired on samples collected from rats after 5/6 nephrectomy or sham operation [1]. There were a total of 147 urine samples, 53 serum samples, and 130 tissue samples, and for each collected sample multiple NMR spectra were recorded using different pulse sequences: 1D nuclear Overhauser effect spectroscopy (NOESY), Carr-Purcell-Meiboom-Gill (CPMG), 2D J-resolved (JRES) including a skyline projection (pJRES), and a quantitative NOESY spectrum using a long relaxation delay (qNOESY). 01–30 (no data for rats 01, 06, 16, 19, 22 or 23) 00–06 (illustrated in Fig. 1) NOESY (01), CPMG (02), JRES (03), qNOESY (04). Rats 1, 6, 16, 19, 22, and 23 died before the end of the experiment, mostly during or immediately after the surgical procedures, and no data is available for these rats
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