Tissue Propagation of Marjoram Plant (in vitro)

Abstract

The experiment was conducted in the plant tissue culture laboratory of the Department of Horticulture and Landscape Engineering, College of Agriculture, University of Kirkuk for the season 2022. to study the propagation of the marjoram plant tissue from tips of branches outside the body. plant parts were sterilized for a period of 10-15 min, water was used to remove dust, and they were sterilized inside the cultivation table in Naocl sodium hypochlorite solution (commercial minor solution) at a concentration of 6% for 8 minutes in order to remove suspended microscopic parts. plant parts were washed 3 times each for 5 minutes. plant parts were cut, and tips of branches were taken, with (1) cm. with kinetin at, 2, 4, 6, 8) mg. L-1. plants were vaccinated at a temperature of 25 + 2 m 0 with an intensity of illumination was 3000 lux, with a light sequence of 16 hours, followed by 8 hours of darkness, for 4 weeks of cultivation. data were taken for the experiment (length of the longest branch) (number of branches) (number of leaves). after replanting the tips of the branches for another 4 weeks of the multiplication process, data were taken at (length of the longest branch cm) number of leaves/plant part). tips of branches. Regarding the root experience, data were taken (percentage of rooting %) (average number of roots/vegetative part) (average root length/cm) (branch length/cm) (number of leaves/vegetative part) full and half concentration of salt from the same soil were added to cultivated on a medium with different concentrations of IBA.