Abstract
Tissue microarray (TMA) helps to study the large number of tissue samples in a single section. In this technique few hundreds to thousands of tissue samples from the different paraffin block can be taken and are precisely put into a recipient paraffin block. TMA helps to amplify the resource material and provides the uniformity in staining conditions without affecting the original donor block. It is also faster and cheaper than conventional staining procedure. TMA helps to perform immunocytochemistry (ICC), fluorescent in situ hybridization (FISH) or RNA analysis in the single section from the recipient block. This chapter presents the method to construct TMA, designing the grid and various applications of TMA in histopathology.
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