Abstract
The proteolipids (PLPs) are abundant components of mammalian CNS myelin. Recombinant DNA methodologies have enabled us to search for evolutionary antecedents of PLP/DM20. Polymerase chain reactions of Torpedo and Squalus brain cDNA were performed with degenerate primers designed according to the mammalian PLP/DM20 sequence. Three DM20-related products (DM alpha, DM beta, and DM gamma) were amplified; no cDNAs containing the PLP-specific segment were found. Regions of the DM alpha and DM gamma are similar to the pore-forming segments of certain ligand-gated channels. In embryonic Squalus CNS, DM alpha and DM gamma appear to be co-expressed with P0. Antiserum raised against Torpedo DM alpha recognizes a protein in mouse CNS myelin, demonstrating that at least one of the newly recognized fish DMs is also in mammals. Our data, as well as that of other laboratories, supports the existence of a ubiquitously expressed proteolipid gene family.
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