Tissue hydrate layer-trigger swollen gelatin-based aerogel hemostatic material with bletilla striata complex active ingredient complex to promote hemostasis

Abstract

Gelatin exhibits excellent solubility at the 37℃ tissue surface due to its affinity for the rich tissue hydrate water. However, its inherent property doesn't facilitate the formation of a robust physical barrier to effectively manage wound hemorrhage and promote hemostasis. To address this challenge, a novel gelatin-based derivative (Ged) with swollen property upon absorbing the tissue hydrate layer was prepared. In this work, a hydrophobic monomer was introduced into gelatin under amide reaction to regulate the interactions between water molecules and gelatin molecular chains. This investigation results showed that Ged exhibited excellent adhesion properties and long-term swollen at 37 °C. In addition, bletilla striata complex active ingredients (Bscai) was employed to the Ged to promote hemostasis capacity, which referred to as Bscai/Ged hemostatic material. In-depth experiments were conducted to investigate the structural characteristics and biocompatibility of Bscai/Ged hemostatic materials, those results revealed that Bscai/Ged hemostatic materials had swift hydrate layer-trigger rate, robust adhesive strength on wet tissue surfaces, outstanding hemostatic properties and facilitating accelerated wound healing. Additionally, in vivo experimental results indicated that the Bscai/Ged hemostatic material could mitigate inflammatory responses, promote collagen deposition and enhance angiogenesis. Overall, Bscai/Ged hemostatic material presents a highly promising candidate for advanced hemostatic material applications.