Tissue fluid pressure, lymph pressure, and fluid transport in rat intestinal villi

Abstract

Tissue fluid pressure ( P t) and lymph pressure ( P l) as well as fluid transport between initial lymphatics and tissue matrix were determined in the villi of the jejunum in vivo and in vitro. When the intestine was absorbing fluid, free tissue fluid as a micro pool appeared in the villi. P t and P l were 2.4 ± 0.7 and 2.9 ± 0.8 mm Hg (mean ± SE), respectively, in the villi in vivo (26 villi of 5 rats) or 0.8 ± 0.4 and 1.2 ± 0.5 mm Hg, respectively, in the villi in vitro (30 villi of 5 rats). P l was significantly ( P < 0.01) higher than P t. When Evans blue in saline was continuously infused into an initial lymphatic, dye rapidly leaked out into the tissue matrix, but when it was infused into the free tissue fluid, dye did not enter the lymphatics but leaked out of the epithelial layer, apparently due to low hydraulic conductance of the tissue matrix to fluid transport in this direction. Furthermore, in the villi with fat absorption, a retrograde flow of chylous lymph out of the villous tips always occurred, indicating that there are large pores at the villous tips to allow free passage of fluid and chylomicrons. From these findings and other evidences, it is inferred that during fluid absorption a fraction of the lymph may be formed by the transport of the luminal fluid directly into the terminal end of the initial lymphatics via large pores at the villous tips presumably by imbibition of the tissue matrix or by lymphatic suction or both.