Abstract
We characterised a tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression in relation to severity of inflammatory infiltration of the gallbladder mucosa in a chronic cholecystitis. We prospectively studied the gallbladder specimens obtained from 54 patients who had undergone cholecystectomy due to chronic calculous cholecystitis and 16 calculosis-free gallbladder specimens obtained from patients who underwent cholecystectomy due to the polyp/polyps as well as in cases of gallbladder injury. To assess TF and TFPI immunoreactivity by immunohistochemistry, the monoclonal anti-human TF and TFPI antibodies were used. The inflammatory infiltration of the gallbladder mucosa was reflected by the number of CD3 and CD68 positive cells. The expression of TF and TFPI differed significantly between the cholecystitis and the control group. Most capillary endothelial cells of the cholecystitis group presented weak expression for TFPI. The mean number of CD3 positive lymphocytes in the cholecystitis group was 18.6 ± 12.2, but the mean number of CD68 positive cells was 29.7 ± 13.9. In the control sections, it was 3.1 ± 1.9 and 8.8 ± 3.9, respectively (P < 0.001). The results of the current study suggest that the tissue procoagulant state found may be engaged in the etiopathogenesis of the cholecystitis.
Highlights
Chronic cholecystitis is characterised by chronic inflammation of a gallbladder mucosa and it is usually associated with gallstones [1]
We prospectively studied the serial cryostat sections of the gallbladder specimens obtained from 54 consecutive patients who had undergone cholecystectomy under the clinical diagnosis of chronic
To the best of our knowledge, we for the first time demonstrated a procoagulant state in gallbladder tissue of the patients with clinically diagnosed chronic calculous cholecystitis
Summary
Chronic cholecystitis is characterised by chronic inflammation of a gallbladder mucosa and it is usually associated with gallstones [1]. In light of recent studies, chronic inflammatory conditions are tightly related to tissue procoagulation state [3]. In this context, tissue factor (TF; CD142) transmembrane receptor and cofactor for clotting factor VII/VIIa have been reported to play a principal role in the initiation of inflammation-induced coagulation [4]. Tissue factor pathway inhibitor (TFPI) provides anticoagulative and anti-inflammatory tissue activity by inhibiting the TF:FVIIa complex and factor Xa [6]. The purpose of this study was to characterise TF and TFPI phenotype expression in relation to severity of inflammatory cell infiltration of gallbladder mucosa
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