Abstract
Tissue engineering produces constructs with defined functions for the targeted treatment of damaged tissue. A complete spinal cord injury (SCI) model is generated in canines to test whether in vitro constructed neural network (NN) tissues can relay the excitatory signal across the lesion gap to the caudal spinal cord. Established protocols are used to construct neural stem cell (NSC)‐derived NN tissue characterized by a predominantly neuronal population with robust trans‐synaptic activities and myelination. The NN tissue is implanted into the gap immediately following complete transection SCI of canines at the T10 spinal cord segment. The data show significant motor recovery of paralyzed pelvic limbs, as evaluated by Olby scoring and cortical motor evoked potential (CMEP) detection. The NN tissue survives in the lesion area with neuronal phenotype maintenance, improves descending and ascending nerve fiber regeneration, and synaptic integration with host neural circuits that allow it to serve as a neuronal relay to transmit excitatory electrical signal across the injured area to the caudal spinal cord. These results suggest that tissue‐engineered NN grafts can relay the excitatory signal in the completely transected canine spinal cord, providing a promising strategy for SCI treatment in large animals, including humans.
Highlights
neural stem cell (NSC), either endogenous or exogenous, because they are prone to differentiate into astrocytes in the post-spinal cord injury (SCI) milieu.[8]
For and ascending nerve fiber regeneration, and synaptic integration with host example, neurons and glia derived from neural circuits that allow it to serve as a neuronal relay to transmit excitatory electrical signal across the injured area to the caudal spinal cord. These results suggest that tissue-engineered neural network (NN) grafts can relay the excitatory signal in the completely transected canine spinal cord, providing a promising grafted human neural stem cells (NSCs) were able to replace lost neurons and glia excised by hemisection surgery, and they functioned as interneurons to reconnect severed neural circuits in a rodent SCI
NSCs were isolated from the hippocampus, and nestin-positive neurospheres were transfected with a lentivirus carrying a TrkC coding sequence
Summary
NSCs, either endogenous or exogenous, because they are prone to differentiate into astrocytes in the post-SCI milieu.[8] When. Spinal cord injury (SCI) causes irreversible tissue loss, NSCs were delivered in a fibrin–thrombin matrix containing including neurons in the gray matter and nerve tracts and trophic factors including brain-derived neurotrophic factor. Zeng Department of Histology and Embryology Zhongshan School of Medicine Sun Yat-sen University Guangzhou 510080, China
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