Abstract

2,3,7,8-Tetrachlorodibenzo- p-dioxin (TCDD) was administered intravenously (i.v.) to adult female Wistar rats as a single dose of 300 ng 14C-TCDD/kg body wt. At different time points after treatment (up to 175 days), TCDD concentrations were measured in different tissues and the activity of ethoxyresorufin O-deethylase (EROD) was measured in the liver microsomes. The results were compared with those observed after subcutaneous (s.c.) application of the same dose of TCDD. Highest TCDD concentrations in the liver (4.5 ± 1.1 ng/g wet wt) were measured 3 hours after i.v.-application, whereas after s.c.-administration of the same dose the maximum concentration in the liver (4.7 ± 0.9 ng/g wet wt) was reached 3 days after application. About 54% of the administered dose of TCDD was found in the whole liver 3 hours after i.v.-application. In adipose tissue, TCDD concentration was found to be maximal 7 days after the injection in both i.v.- and s.c.- treated groups (0.85 ± 0.06, and 0.82 ± 0.07 ng/g wet wt, respectively). Hepatic EROD activity was maximal 1 day after the i.v.-injection and 7 days after the s.c.-injection. EROD activities correlated well with target (hepatic) tissue concentrations of TCDD, regardless of the route of administration.

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