Abstract
AbstractThe seed‐borne nature of Peronospora parasitica in cabbage cultivar is well established. Pure axenic growth of downy mildew of cabbage is established in vitro using modified Murashige and Skoog medium. The tissue culture technique is used to test the viability of seed borne mycelium of P. parasitica in cabbage. The percentage of seeds showing viable mycelium varied from cultivar to cultivar and a direct correlation was noticed between embryo infection and seed transmission in callus. A similar correlation was established with the seedling symptom test. The tissue culture technique to test the viability of downy mildew mycelium in cabbage was a quicker and more reliable method than the seedling symptom test. The possibility of adopting the technique by quarantine stations for seed health testing and for the in vitro studies of P. parasitica and other host systems in crucifers is discussed.
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