Abstract
We have used tissue culture to study the process of phagocytosis of rod outer segments (ROS) by normal and dystrophic rat pigment epithelial (PE) cells. These cells in culture retain some of their in vivo capabilities, viz. (a) the ability to distinguish between light and dark adapted ROS ( Hall, 1978); (b) PE cells from dystrophic rats have a reduced phagocytic capability in comparison with PE cells from normal rats ( Hall, 1979). However, we do not know whether these functions are partially reduced in tissue culture, or to what extent other in vivo properties are adversely affected by removing the cells from the eye. We have thus studied the growth of PE cells on different supporting media in order to find conditions under which these cells morphologically and functionally resemble PE cells in vivo. As a first approximation, we have compared the parameters of plating efficiency, morphological appearance, and the ability to phagocytize and digest ROS, of PE cells grown on different media. Not all parameters have been measured for each supporting medium.
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