Abstract
Abstract The influence of explant (shoot-tip, node, or internode), growth regulators, liquid pretreatment, and Murashige and Skoog (MS) salt concentrations on callus, shoot, and root production was determined for Osage-orange [Maclura pomifera (Raf.) Schneid]. Shoots proliferated from both shoot-tip (three shoots/explant) and nodal (two shoots/explant) sections, but not from internodes. Optimum growth regulator concentrations for shoot proliferation were 0.5 µm IBA, 6 µm BA, and 3 µm GA. A 48-hr pretreatment of liquid-modified MS medium (MMS) did not enhance shoot proliferation. Internode sections produced more callus than shoot-tip or nodal sections with the highest production (620 mm3) on MMS medium plus 0.5 µm IBA and 4 µm BA. Micropropagated shoots rooted best on half-strength MS salts plus 2.5 µm IBA. Plant-let survival in the greenhouse after 2 months was > 70%. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA), 1H-indole-3-butyric acid (IBA), gibberellic acid (GA).
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