Tissue culture of Lavandula angustifolia L.

Abstract

Objective: The study is to establish the key techniques of tissue culture of Lavandula angustifolia L. Method: Using stem with buds of Lavandula angustifolia L. as explant, the effect of disinfection time on contamination rate, types and ratio of plant regulator on propagation index and rooting, types of transplanting substance on plantlet survival rate were studied. Results and conclusion: The proper disinfection time of 0.1 % HgCl2 was 15min, by which the survival and contamination rate of plantlets respectively were 83.3 % and 25.0 %; MS +1.0mg/L BA + 0.3 mg/L IBA was the proper medium for propagation, which propagation index was 4.92; White + 0.4 mg/L NAA was the proper medium for rooting, which rooting rate and the average number of roots per plantlet respectively were 66.7% and 9.4; the survival rate of plantlets on transplanting substance which were peat and perlite mixed with an equal volume was 66.7 %.