Abstract
Ficus carica or commonly known as fig plant is a deciduous plant originated from southwest Asia and eastern Mediterranean. It has many benefits in medical field especially to treat diseases such as rheumatism and haemorrhoids due to its high laxative activity effect. The main objective of this study is to develop in vitro clonal propagation method for rapid production of Ficus carica variety BTM-6 plantlet using different plant growth regulators (PGRs) through shoot induction and multiplication, rootings and subsequent establishment in soil following acclimatization. Surface sterilisation of the explant was done using sodium hypochlorite as the disinfectant. Pre-treatment of the explants with carbendazim successfully reduced the occurrence of fungal contamination. To investigate the effect of plant growth regulators on shoot induction, explants were cultured in different concentrations of PGRs either singly or in combination. No shoot and root inductions were observed but calli were successfully induced on MS medium containing 2 mg/l BA only, 2 mg/l BA in combination with 0.5 mg/l NAA and MS media supplemented with 0.5 mg/l BA in combination with 0.5 mg/l NAA. A further in-depth study using other different types of plant growth regulators at various concentrations is required in order to establish a complete tissue culture protocol of this particular plant species.
Highlights
Ficus carica or commonly called as fig is a deciduous plant from the family of Moraceae and originated from southwest Asia and eastern Mediterranean
Figs are among plant species that have been mentioned in the Holy Quran besides olives, grapes, pomegranates and dates
To overcome the problem with fungal contamination, explants were pre-treated with fungicide. 0.2% carbendazim was used as a pre-treatment
Summary
The matured fruit of fig has tough skin and will crack upon ripeness. It has mass-bound seeds with outer ring in white and maroon red jelly-like flesh. Abu ad-Darda’ A.S narrated that the Prophet (PBUH) said: Quick Response Code “If I could say that a fruit was sent down from Heaven (to earth), I would say it is figs, because the Heaven's fruit has no stones. Eat it, as it cures hemorrhoids and it is useful for treating gout" (Shahih al-Bukhari)
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