Abstract
The author describes a method used for culturing the tissue of intact ascending rabbit aorta, as well as of ascending rabbit aorta with experimental atherosclerosis (without adventitia). Bits of these tissues were cultured in Carrel's flasks containing homologous nutritive ingredients. A method of culturing the tissue of human aorta, resected during the operation for coarctation of the aorta is also described. In culturing the rabbit aorta with experimental atherosclerosis in the presence of normal serum the degree of cellular adiposity was less marked than that observed in the cultures of intact aorta at the corresponding periods. In culturing the atherosclerotic rabbit aorta with hyperlipemic serum there was a much greater degree of lipoidosis and a greater intensity of cellular degeneration than in the cultures of the same aorta grown in the presence of normal serum.
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