Tissue Culture and Rapid Propagation of Rare Plant in Codariocalyx motorius(Houtt.)

Abstract

The aseptic seedings of Codariocalyx motorius(Houtt.)Ohashi were the materials of our study on issue culture and rapid propagation,MS and 1/2MS were the basic mediums.Impact of different conditions on the rapid propagation of C.motorius(Houtt.)was discussed and a tissue culture system of C.motorius(Houtt.)was initially established,the best callus adventitious and bud differentiation medium was MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1+sugar 30 g·L-1+VC 2.0 mg·L-1,the best medium for the shoot regeneration was MS+6-BA 2.0 mg·L-1+NAA 0.05 mg·L-1,the best rooting medium was 1/2MS+NAA 0.2 mg·L-1+IAA 0.5 mg·L-1.