Abstract
Portions of previously reported pesticide methods have been uniquely combined to provide a method for the simultaneous determination of several pesticides and their metabolites at low levels in 2-g portions of animal or human tissues. This method, developed for the toxicologist, permits the analysis of a single rat brain for storage and distribution studies and of a human fat biopsy sample for assessment of pesticide exposure. The sample preparation consists of extraction, concentration, and a cleanup by a liquid-solid chromatographic column. Heptachlor epoxide, p, p′-DDE, o, p′-DDT, dieldrin, and p, p′-DDT, in the prepared sample are separated and determined simultaneously by gas chromatography using an electron capture detector. A 5-foot glass column utilizing a silicone liquid phase was operated at 155°C to provide a separation of pesticide residues which were of particular interest in a study of human fats from four geographical areas of the United States. The method, providing quantitative recoveries from tissues, will detect a minimum of 0.01 ppm, p, p′-DDT and even lower concentrations of other pesticides.
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