Abstract
The pioneering attempts of G. Haberlandt (1902) to culture plant cells did not succeed but with the refinement of the in vitro techniques and tissue culture media and the discovery of plant growth regulators it has been possible to establish various types of tissue cultures, starting from embryonic, meristematic and mature tissues. Broadly, there are two types of tissue cultures: (i) unorganized (callus and suspension cultures), and (ii) organized (root cultures, shoot tip cultures, embryo cultures etc.). Organized tissue cultures are dealt with in some of the following chapters. This chapter describes unorganized tissue cultures.
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