Tip 2 Diyabetik Sıçanlarda Kurkuminin Streptozosin Kaynaklı Pankreatik Adacık Hasarına Karşı Koruyucu Etkisi: Deneysel Bir Çalışma

Abstract

Objective: The aim of study of was to determine the damage caused by streptozocin (STZ)-induced Type 2 diabetes in pancreatic islet in rats and the effect of curcumin on this damage. Material and Methods: A total of 32 (250-320 g) Wistar albino male rats were used. Rats were randomly divided into 4 groups as Control (C), Diabetes (DM), Curcumin (CUR), and Diabetes+Curcumin (DM+CUR) (n=8). Histopathological, stereological and immunohistochemical analyzes were used to determine the damage caused by type 2 diabetes in the pancreatic tissue and the effect of curcumin on this damage. Results: In the histopathological evaluation of pancreatic tissues, in pancreatic sections of DM group rats, the islets were smaller and their borders were irregular, and degenerative and necrotic changes were observed in the cells. In the DM+CUR group, it was determined that these degenerative changes in the islets of Langerhans and cells were alleviated. In the stereological analysis, it was determined that the islet volume, the ratio of islets to the pancreas and the number of beta cells in the DM group were statistically significantly decreased compared to the other groups, and it was found to be higher in the DM+CUR group than in the DM group (p<0.05). In the immunohistochemical evaluation, the C and CUR groups showed similar staining with Ki67, whereas the staining was less in the diabetic group. In the diabetes group treated with curcumin, it was found that curcumin attenuated the diabetes- related Ki67 reduction. Conclusion: In this study, it was determined that curcumin reduced the damage in the islets and beta cells in the pancreas of rats with diabetes mellitus with STZ and provided a significant protection in the pancreatic tissue.