Abstract
This in vitro study was designed to evaluate the effect of sol-gel derived TiO2 coating on blood coagulation, blood protein adsorption, and platelet response on zirconia surfaces. Square-shaped zirconia (n=96) (10x10x2 mm) was cut, ground, sintered, and finally cleansed ultrasonically in each of acetone and ethanol for 5 minutes. Three experimental groups (n=32) were fabricated: (a) zirconia coated with sol-gel derived TiO2, (b) zirconia coated with sol-gel derived TiO2 and treated with ultraviolet (UV) irradiation for 1 hour, and (c) non-coated zirconia as control. The coatings were prepared from tetraisopropyl orthotitanate solution by dip-coating. The thrombogenicity of the specimens was evaluated using a whole blood kinetic clotting time method where the extent of blood clotting was evaluated at 10, 20, 30, 40, 50, and 60 minutes (n=4/time point, total n=24/group). Scanning electron microscope images were taken to observe platelet morphologies after 1-hour incubation with platelet-rich plasma (PRP) (n=5/group). Surface characteristics were visualized using atomic force microscopy (n=1/group). Adsorption of plasma proteins and fibronectin on each surface was studied by gel electrophoresis (n=2/group). Significant differences were observed in blood coagulation between the test groups at 20-, 30-, 40-, and 50-minute time points (p<0.005). UV treated TiO2 coated specimens showed fastest blood coagulation followed by TiO2 coated and non-coated specimens. Furthermore, platelets appeared at a higher activation state on coated specimens. Gel electrophoresis revealed no difference in protein adsorption among the experimental groups. In summary, TiO2 coatings promoted blood coagulation, and it was further enhanced by UV treatment, which has the potential to hasten the wound healing process in vivo.
Highlights
Zirconia is being an increasingly used material in prosthetic dentistry [1]
TiO2 coated+UV treated specimens represented the highest extent of blood clotting at all the forenamed time points (p
The time taken for blood coagulation, in other words the total clotting time, was almost 30 minutes for the TiO2 coated+UV treated specimens followed by 40 minutes and 50 minutes for the TiO2 coated and non-coated control specimens, respectively
Summary
The advantageous properties of zirconia as a biomaterial are a consequence of addition of yttria (Y2O3) to zirconia (ZrO2) crystals that yields what is commonly known as Yttria-stabilized Tetragonal Zirconia Polycrystal (Y-TZP) [2]. Once sintered, it has high bending strength and properties that are similar to stainless steel alloys [1, 2]. Zirconia abutments possess better light dynamics and the final prosthesis is more esthetic to the human eye [1]. It has been found to elicit less plaque accumulation and provoke a weaker inflammatory response than titanium [1, 3, 4]
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