Time-resolved two-photon excitation fluorescence spectroscopy and microscopy using a high repetition rate streak camera

Abstract

We present a time-resolved two-photon excitation fluorescence spectroscopy and a simultaneous time-and spectrum-resolved multifocal multiphoton microscopy system that is based on a high repetition rate picosecond streak camera for providing time-and spectrum-resolved measurement and imaging in biomedicine. The performance of the system is tested and characterized by the fluorescence spectrum and lifetime analysis of several standard fluorescent dyes and their mixtures. Spectrum-resolved fluorescence lifetime images of fluorescence beads are obtained. Potential applications of the system include clinical diagnostics and cell biology etc.